The Km of a Michaelis Menten enzyme for a substrate is 1*10^-4M. At substrate conc. of 0.2M vo=43uM/min for a certain enzyme concentration. However with a substrate conc. of 0.02, vo has the same value. Show that this observation is accurate?

Michaelis-Menten Expression (MME): V0= Vmax*[S] / (Km + [S]) ——————————————————— We know regardless of [S], Km= 1x10^-4 for this enzyme. Observation A ([S] = 0.2M), MME: 4.3x10^4 = 0.2*Vmax_a / (1x10^-4 + 0.2) Observation B ([S] = 0.02M), MME: 4.3x10^4 = 0.02*Vmax_b / (1x10^-4 + 0.02) ——————————————————— Therefore: 0.2*Vmax_a / (1x10^-4 + 0.2) = 0.02*Vmax_b / (1x10^-4 + 0.02) If you simplify, you get: Vmax_a/Vmax_b = 0.2001/0.201 = 1.00 (3.s.f) Therefore the Vmax is almost identical in both circumstances. ——————————————————— Both circumstances use the same Vmax and Km values to achieve the same V0 therefore the observation is feasible and accurate. We can derive from this that substrate concentrations at, and over, [S]=0.02M do not result in an increase in V0; the Vmax has been reached.

Ali Rajani

4.7k students helped

Biology Tutor • London

Tutors on Scoodle who can help with Biology

Verified

Yousaf Caan

DBSOnline lessons

Experienced tutor and undergraduate at Newcastle University

Hi, I am a 2 year medicine student who has a experience of teaching and tuition. My dream is to become a lecturer and so have taken on many teaching roles within my university. I believe the best way to learn it’s through doing repetitive practise of questions

Lubna Maqbool

DBSOnline lessons

Qualified and experienced teacher

Introduction I am a fully qualified Science teacher with a specialism in Biology. I am passionate about helping others achieve their academic goals in a supportive and intellectually stimulating environment. Education I studied Biomaterials Science at the University of Birmingham. Later, I pursued a Master's in Infection and Immunity at the University of Leicester for which I was awarded the best student prize. I completed my PGCE from the University of Warwick and undertook a master's level project on misconceptions in science. Teaching experience Collectively, I have 17 years of experience tutoring face to face and online. Additionally, I have worked in schools and colleges across the West Midlands for the past 9 years. I have taught GCSE Science, BTEC Applied Science, A-level Biology and A-level Chemistry, covering the OCR, AQA and Edexcel specifications. In my position as the Head of Department I coordinated and delivered study skills sessions which included note taking and memory techniques to boost the performance of my students. I have been monitored by OCR for the practical endorsement for which I received positive feedback. Over the years I have helped students achieve or exceed their target grade. Three of the students I tutored this academic year have progressed from an E grade and below to an A grade in their school mocks. Tutoring approach I have a holistic approach to teaching ensuring students make rapid progress. My aim is to give my students the skills and tools to become confident, independent and reflective learners. Many students fail to perform in examinations to their best ability , despite putting in many hours of revise. My lessons focus on providing effective exam techniques and the key points required by the examination board. Each lesson is tailored entirely to the individual learners` requirements.

£30Per hour

230Tutor score

Verified

Muhammad Saifuddin

Online lessons

Medical Sciences Student, 2+ years of experience at a licensed tuition centre :)

Studying Medical Sciences at The University of Edinburgh has been an experience that has not only helped me understand the importance and value of education, but also taught me to have pride for the achievements that you accomplish at every stage of life; emanating from an individuals hard work and effort, this in itself is a great motivator. Having a years worth of experience tutoring KS3, GCSE and A level students at a licensed tuition centre, I realised my personal aim is not only to aid my tutees in achieving their fullest potential but allowing them to go beyond so that they become ever-gratifying to their dedication, and not only walk away from their schooling years with a sheet of paper that rewards their diligence, but also a work ethic and skill set that opens up many opportunities in life. Academia addresses more than just memorising the examinable materials. Every subject tests different degrees of critical thinking, application of knowledge and strategic answering. Recognising the importance of being able to do so is vital, both in and out of the exam hall, in order to achieve success. I aim to provide my tutees with a stress relieving teaching experience that is engaging and effective, all while honing their thinking skills, alongside making learning new content a passion rather than a chore. My teaching methods ensure that the child is always looking forward to their next lesson, which enforces a positive mindset - vital to effective learning!

Mohammed Hussin

Online lessons

Maths/Science tutor with FREE TRIAL

Hello I am an experienced tutor currently studying Chemistry, Biology and Maths A levels in a reputable college. I am working part time in a well known tutoring centre providing face to face maths lessons to students of primary and secondary schools. I have an extensive experience of working with children and I am also DBS checked. I can engage positively with my students and being a student myself, help me to understand the need to tailor lessons for those who lacks motivation or require additional guidance. I am currently only providing online lessons and therefore offering a free trial sessions during which it can be decided whether it would work for the students. Please contact me via email/text/call to arrange a free trial. Thanks

£12Per hour

210Tutor score

Verified